tbe urea gel (Thermo Fisher)
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Tbe Urea Gel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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Centrifugation:Article Title: A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation Article Snippet: .. Precipitated RNAs were pelleted by centrifugation at 16,000× g for 20 min, washed with 300 μl 70% ethanol, and resuspended in 10 μl 2× Purification:Article Title: DNA glycosylases provide antiviral defence in prokaryotes Article Snippet: Five microlitres of each was mixed with loading dye and loaded onto a 10% TBE gel (Invitrogen, EC62755BOX) and electrophoresed at 140 V for 35 min. Gels were stained with 2 μg ml −1 ethidium bromide for 20 min, extensively rinsed with distilled water (3× for 10 min each), and then scanned using a ChemiDoc MP imager (Bio-Rad) set to UV trans illumination and the machine’s 605/50 filter to detect ethidium bromide (Extended Data Fig. ) or using the Amersham ImageQuant 800 set to UV fluorescence (all other relevant figures). .. For Urea-PAGE gels, eluted samples were first denatured by mixing 5 μl of purified sample with 5 μl of 2× Article Title: DNA glycosylases provide antiviral defence in prokaryotes. Article Snippet: Five microlitres of each was mixed with loading dye and loaded onto a 10% TBE gel (Invitrogen, EC62755BOX) and electrophoresed at 140 V for 35 min. Gels were stained with 2 μg ml−1 ethidium bromide for 20 min, extensively rinsed with distilled water (3× for 10 min each), and then scanned using a ChemiDoc MP imager (Bio-Rad) set to UV trans illumination and the machine’s 605/50 filter to detect ethidium bromide (Extended Data Fig. 3h) or using the Amersham ImageQuant 800 set to UV fluorescence (all other relevant figures). .. For Urea-PAGE gels, eluted samples were first denatured by mixing 5 μl of purified sample with 5 μl of 2× Imaging:Article Title: DNA glycosylases provide antiviral defence in prokaryotes Article Snippet: Five microlitres of each was mixed with loading dye and loaded onto a 10% TBE gel (Invitrogen, EC62755BOX) and electrophoresed at 140 V for 35 min. Gels were stained with 2 μg ml −1 ethidium bromide for 20 min, extensively rinsed with distilled water (3× for 10 min each), and then scanned using a ChemiDoc MP imager (Bio-Rad) set to UV trans illumination and the machine’s 605/50 filter to detect ethidium bromide (Extended Data Fig. ) or using the Amersham ImageQuant 800 set to UV fluorescence (all other relevant figures). .. For Urea-PAGE gels, eluted samples were first denatured by mixing 5 μl of purified sample with 5 μl of 2× Article Title: DNA glycosylases provide antiviral defence in prokaryotes. Article Snippet: Five microlitres of each was mixed with loading dye and loaded onto a 10% TBE gel (Invitrogen, EC62755BOX) and electrophoresed at 140 V for 35 min. Gels were stained with 2 μg ml−1 ethidium bromide for 20 min, extensively rinsed with distilled water (3× for 10 min each), and then scanned using a ChemiDoc MP imager (Bio-Rad) set to UV trans illumination and the machine’s 605/50 filter to detect ethidium bromide (Extended Data Fig. 3h) or using the Amersham ImageQuant 800 set to UV fluorescence (all other relevant figures). .. For Urea-PAGE gels, eluted samples were first denatured by mixing 5 μl of purified sample with 5 μl of 2× Fluorescence:Article Title: DNA glycosylases provide antiviral defence in prokaryotes Article Snippet: Five microlitres of each was mixed with loading dye and loaded onto a 10% TBE gel (Invitrogen, EC62755BOX) and electrophoresed at 140 V for 35 min. Gels were stained with 2 μg ml −1 ethidium bromide for 20 min, extensively rinsed with distilled water (3× for 10 min each), and then scanned using a ChemiDoc MP imager (Bio-Rad) set to UV trans illumination and the machine’s 605/50 filter to detect ethidium bromide (Extended Data Fig. ) or using the Amersham ImageQuant 800 set to UV fluorescence (all other relevant figures). .. For Urea-PAGE gels, eluted samples were first denatured by mixing 5 μl of purified sample with 5 μl of 2× Article Title: DNA glycosylases provide antiviral defence in prokaryotes. Article Snippet: Five microlitres of each was mixed with loading dye and loaded onto a 10% TBE gel (Invitrogen, EC62755BOX) and electrophoresed at 140 V for 35 min. Gels were stained with 2 μg ml−1 ethidium bromide for 20 min, extensively rinsed with distilled water (3× for 10 min each), and then scanned using a ChemiDoc MP imager (Bio-Rad) set to UV trans illumination and the machine’s 605/50 filter to detect ethidium bromide (Extended Data Fig. 3h) or using the Amersham ImageQuant 800 set to UV fluorescence (all other relevant figures). .. For Urea-PAGE gels, eluted samples were first denatured by mixing 5 μl of purified sample with 5 μl of 2× Polyacrylamide Gel Electrophoresis:Article Title: Purification of DNA oligonucleotides to improve hybridization chain reaction performance. Article Snippet: Hybridization Chain Reaction (HCR) is a technique to generate a linear polymerization of oligonucleotide hairpins, used in multiple molecular biology methods.. The HCR reaction is dependent on every hairpin being metastable in the absence of a triggering oligonucleotide and that every hairpin can continue the polymerization, which puts a strong demand on oligonucleotide quality.. We show how further purification can greatly increase polymerization potential. |
